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Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.

Identifieur interne : 001131 ( Main/Exploration ); précédent : 001130; suivant : 001132

Validation of reference genes for RT-qPCR analysis in Burkholderia pyrrocinia JK-SH007.

Auteurs : Bin-Yan Wu [République populaire de Chine] ; Jian-Ren Ye [République populaire de Chine] ; Lin Huang [République populaire de Chine] ; Ling-Min He [République populaire de Chine] ; De-Wei Li [États-Unis]

Source :

RBID : pubmed:27725176

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English descriptors

Abstract

Burkholderia pyrrocinia strain JK-SH007 isolated from poplar stems plays a highly significant role in the growth promotion and the biocontrol of poplar canker during colonization in poplar. In this research, the ideal reference gene was filtered and determined for the transcript normalization. Additionally, the expression of pyrG under all four conditions was relatively stable in B. pyrrocinia JK-SH007.

DOI: 10.1016/j.mimet.2016.10.004
PubMed: 27725176


Affiliations:


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